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. 2015 Oct 1;142(19):3383–3393. doi: 10.1242/dev.125393

Fig. 1.

Fig. 1.

Wnt/β-catenin activity in stromal keratocytes is inversely correlated to corneal epithelium stratification. (A-E) Corneal epithelium stratification is a postnatal event. From P1 to P7 the mouse epithelium consists of 1-2 cell layers. Just before eyelid opening at P10, the epithelium begins to stratify. At P15 it increases to 4-5 cell layers and, by P21, there are 5-6 layers. (F-J) Expression of the active form of β-catenin (immunofluorescence, green) is found in the corneal epithelium and stroma from P1 to P21. Note that the β-catenin expression pattern did not change in the corneal epithelium from P1 to P21, but exhibited a dynamic change in the stroma. Nuclear localization of β-catenin was detected in the stroma at P1 to P7 and gradually decreased through P21. Boxed regions (F,G) are shown at higher magnification in insets. Blue, cell nuclei (DAPI). (K) Schematic representation of the reduction of Wnt/β-catenin activity coinciding with epithelial stratification. Scale bars: 20 μm.