Fig. 8.
Ctnnb1 ablation in keratocytes results in Smad1/5 phosphorylation and enhances p63 expression. (A-F) Immunofluorescence staining showed that at E16.5 more p63-labeled basal cells and more pSmad1/5-labeled basal cells were detected in Ctnnb1cKO mutant corneal epithelium than in the littermate controls. Note that p63 expression was increased in the mutant and that this colocalized with Smad1/5 phosphorylation. The dashed line delineates the corneal epithelium layer. (G) Statistical analysis of the immunofluorescence staining showed that Smad1/5 phosphorylation and p63 expression were upregulated in the mutant. *P<0.05, **P<0.01. Mean±s.e.m.