Fig. 4.
Bivalency marks H3K27me3 and H3K4me3 are maintained in perinatal supporting cells. Schematic shows the locations across the Atoh1 locus (sites 1, 2 and 3; triangles) analyzed by µChIP qPCR for the presence of H3K27me3 and H3K4me3. Hair cells (Atoh1-GFP transgenic mice) and supporting cells (p27Kip1-GFP transgenic mice) were FACS-purified from P1 and P6 mice. H3K27me3 levels remain relatively unchanged in P6 supporting cells, relative to P1. H3K4me3 is also maintained at similar levels in both P1 and P6 supporting cells. Moreover, both marks are maintained at higher levels in supporting cells at P1 and P6 compared with hair cells. Red bars, H3K27me3; green bars, H3K4me3. Data are presented as percentage of input. Results are mean±s.e.m., *P<0.05 (n=3).