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. 2015 Oct 15;142(20):3478–3487. doi: 10.1242/dev.127951

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© 2015. Published by The Company of Biologists Ltd

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Fig. 3. — lin-28 is required in adult ISC lineages to promote ISC number. (A) Number of intestinal tub-Gal4, UAS-GFP-labeled control (+), lin-28^Δ1 (Δ1) and UAS-InR, lin-28^Δ1 (U-InR, Δ1) clones containing ISCs and present at 5, 14 and 27 days after clone induction (ACI). (B,C) Quantification of Dl+ ISC (B) and total cell (C) numbers in tub-Gal4, UAS-GFP-labeled control (+), lin-28^Δ1 (Δ1) and rescued lin-28^Δ1 (Δ1, RC) clones analyzed 5 or 12 days ACI. n, number of clones analyzed. (D) Numbers of cells per tub-Gal4, UAS-GFP-labeled control (+) and lin-28^Δ1 (Δ1) clones analyzed 5 (top) and 12 (bottom) days ACI. (E-G) Representative images of tub-Gal4, UAS-GFP-labeled control (E), lin-28^Δ1 (F) and rescued lin-28^Δ1 (G) ISC clones stained for GFP (green), ISCs (Delta, red) and DAPI (blue). Clone size (dotted line), ISCs (black asterisks) and cell number are indicated. Scale bar: 5 μm.