FIG 4 .

N-WASP and actin are required for E. chaffeensis entry and infection. (A, C) E. chaffeensis (Ech) load in DH82 cells pretreated for 30 min with wiskostatin (0.1, 1, or 10 µM) (A) or with wiskostatin (10 µM) or cytochalasin D (10 µM) (C); in each case, cells were then incubated with E. chaffeensis for 50 h and the bacterial load was determined by qPCR. (B) Numbers of internalized E. chaffeensis bacteria at 4 h p.i. in GFP–N-WASP- or GFP-WA-transfected RF/6A cells at 48 h posttransfection. Quantification of E. chaffeensis in 100 cells. (A to C) Data represent the mean results and standard deviations from triplicate samples and are representative of three independent experiments. *, P < 0.05.