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. 2015 Nov 4;6:967. doi: 10.3389/fpls.2015.00967

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Copyright © 2015 Gawehns, Ma, Bruning, Houterman, Boeren, Cornelissen, Rep and Takken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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One SIX2 knockout is reduced in virulence. Susceptible tomato seedlings were inoculated with wild-type Fol (Fol007) or strains in which SIX2 was deleted (ΔSIX2#1-3). As controls mock-inoculated plants were used (mock) or one transformant (ect#1), in which the SIX2 deletion construct was integrated ectopically. Average plant weight of 15 plants was plotted against the average disease index of the same plants. In ΔSIX2#1 pathogenicity was impaired as shown by the increased plant weight as compared to infection with Fol007 and the ectopic transformant. Error bars represent the standard error. Clustering is based on an ANOVA (Fisher PLSD significant at 95%) using either the disease index (solid line) or plant weight (dashed line).