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. 2015 Nov 4;6:963. doi: 10.3389/fpls.2015.00963

FIGURE 1.

FIGURE 1

The absence of ARS1 leads to ABA-hypersensitive seed germination. (A) T-DNA insertions in the ars1 mutant alleles. (B) RT-PCR analysis of ARS1 expression in WT, ars1-1, ars1-1+Vector, and ars1-1+ARS1 plants. TUBULIN2 serves as a control for RNA integrity. (C) Comparison of seed germination between the WT, ars1-1, ars1-1+Vector, and ars1-1+ARS1 plants exposed to 0 or 1 μM ABA. The photograph shows Arabidopsis seedlings after 5 days of ABA treatment. (D) Expression of ARS1 in WT Col-0 and ars1 allelic mutants determined by RT-PCR. TUBULIN2 serves as a control for RNA integrity. (E) Comparison of seed germination between the WT Col-0 and ars1 mutants exposed to 0 or 1 μM ABA. The photograph shows Arabidopsis seedlings after 5 days of ABA treatment. (F) Quantification of green cotyledons in WT Col-0 and ars1 mutants grown on various concentrations of ABA for 5 days. The data represent the means ± SE of three independent experiments, with 50 seeds per experiment.