Analysis of the effect of temperatures on expression of pETTm0548 (recombinant catalytic subunit of TmAHAS) using SDS-PAGE (15%). Lane 1, CFE, 18 °C; lane 2, CFE 24 °C; lane 3, heat-treated CFE, 24 °C; lane 4, CFE 30 °C; lane 5, heat-treated CFE, 30 °C; lane 6, CFE, 37 °C; heat-treated CFE 37 °C; M: BLUeye pre-stained protein ladder (Froggibio, ON, Canada), the arrows indicate the position of the recombinant protein band (calculated molecular weight 65.5 kDa); 40 µg of the protein loaded per lane.