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. 2015 Oct 3;5:489–497. doi: 10.1016/j.dib.2015.09.018

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — Analysis of the effect of heat-treatment on purification of pETTm0548 (recombinant catalytic subunit of TmAHAS) using SDS-PAGE (12.5%). Lane 1, CFE with no heat-treatment; lane 2, CFE, 30 min at 70 °C; lane 3, CFE, 60 min at 70 °C; lane 4, CFE, 90 min at 70 °C; lane 5, CFE with no heat-treatment; lane 6, CFE, 30 min at 80 °C; lane 7, CFE, 60 min at 80 °C; lane 8, CFE, 90 min at 80 °C; M: BLUeye pre-stained protein ladder (Froggibio, ON, Canada), the black arrow indicate the position of the recombinant protein band (calculated molecular weight 65.5 kDa); approximately 30 µg of the protein loaded per lane.