Fig. 6.

Effect of heat-treatment of crude cell extract of T. maritima on AHAS activity. The CFEs were heat-treated at either 70 °C or 80 °C and data were collected by assaying the AHAS activity at different time points. The relative activities were calculated compared to the sample heat treated for 1 h at each temperature. A relative activity of 100% was considered as the highest specific activities measured at each temperature after 1 h of heat-treatment (8.0 U/mg and 8.7 U/mg at 70 and 80 °C). Vertical columns indicate the protein concentration and scatter plots are the relative AHAS activity.