Fig. 9.

Gel-filtration chromatography of AHAS subunits. Different preparations were loaded on gel-filtration column: (A) catalytic subunit, (B) regulatory subunit, and (C) reconstituted holoenzyme. The numbers indicate the apparent molecular masses of the proteins eluted in each peak. For reconstitution experiment a molar ratio of 1:10 of catalytic subunit to regulatory subunit was mixed together and incubated at room temperature for 1 h. The mixture then was loaded on the size-exclusion column to determine the oligomeric state.