Table 2.

Survey of heat-precipitation temperatures for some recombinant hyperthermophilic proteins expressed in E. coli.

The recombinant protein	Native organism	Heat-precipitation	Topta(°C)	Reference
Glutaredoxin-like protein	Pyrococcus furiosus	65 °C for 10 min	100	[8]
The HU protein	Thermotoga maritima	80 °C for 20 min	80	[9]
Phosphoglycerate kinase	Thermotoga maritima	60 min at 80 °C	80	[10]
ADP-dependent phosphofructokinase	Pyrococcus furiosus	30 min at 80 °C	100	[11]
Chemotaxis protein	Thermotoga maritima	80 °C for 10 min	80	[12]
Maltose-binding protein	Thermotoga maritima	75 °C for 30 min	80	[13]
Carboxylesterase	Sulfolobus sulafataricus	75 °C for 30 min	80	[14]
Glyceraldehyde-3-phosphate dehydrogenase	Pyrococcus woesei	90 °C for 30 min	100–103	[15]
Glyceraldehyde-3-phosphatdee hydrogenase	Thermotoga maritima	Inactive proteinb	80	[16]
Xylose isomerase	Thermotoga maritima	90 °C for 2.5 h	80	[17]
l-arabinose isomerase	Thermotoga neapolitana	85 °C for 15 min	80	[18]
Alcohol dehydrogenase (adhC)	Pyrococcus furiosus	80 °C for 30 min	100	[19]
a-l-arabinofuranosidase	Thermotoga maritima	80 °C for 30 min	80	[20]
6-phosphogluconate dehydrogenase	Thermotoga maritima	90 °C for 30 min	80	[21]

^aOptimum growth temperature of the native hyperthermophilic organism.

^bThe heat-treated purified recombinant protein was inactive.