Skip to main content
. 2015 Oct 9;5:481–488. doi: 10.1016/j.dib.2015.09.043

Fig. 1.

Fig. 1

(A) MAL-I lectin staining followed by cell imaging. Cells were treated for 2 h in the presence of Neu5Ac (10 mM) under nutrient deprivation and stained for 30 min with FITC-labeled MAL-I lectin at concentration of 5 µg/mL (green fluorescence). Cells were further treated with 1 µg/ml RNase A and the nuclei were counter-stained with TO-PRO-3 (red fluorescence), Images are shown at 15 µm magnification. The white arrow represents MAL-I binding of cell surface glycans. © The Delta Vision Elite Imaging System Core Facility at Herbert Wertheim College of Medicine, Florida International University, Miami (File date: 13-12-2012). (B) Blow up of MAL-I staining of MDA MB231 cancer cell showing cell surface glycan sialylation (green region).