Fig. 4.
Effect of antagonists (10 µM each) on the recruitment of βarr. Cells were treated as described in Materials and Methods. Each analyzed ligand was examined for the induction of βarr1 (gray) and βarr2 (black) recruitment. Unless otherwise noted, racemic mixtures were analyzed. (A) Ligands are presented with an uninterrupted y-axis to show relative differences from the reference ligand (R)-ISO. (B) The y-axis is interrupted to show ligand effects in detail. Statistical analysis of antagonist effects compared with solvent control yielded no significance except for the β3AR agonist ICI 215,001, which is a very week inverse agonist concerning βarr2 recruitment. (N = 4; data ± S.D.; two-way analysis of variance with Bonferroni post-test; *P < 0.05).