Figure 7. The effect of gga-19b-3p overexpression on the proliferation of preadipocytes and the differentiation of adipocytes, *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the control group.

(A) the number of preadipocytes was identified using the CCK-8 kit (n = 8), *P < 0.05 vs. the control group. (B) Differentiation of adipocytes was identified by Oil Red O staining (n = 3), ***P < 0.01 and ***P < 0.001 vs. the control. (C) Morphological changes and lipid deposition induced by 50 nM of the gga-19b-3p mimic in preadipocytes during in vitro differentiation (inverted microscope, 400×). lipid droplet, stained with Oil Red O, accumulated as more and larger locules in cells exposed to 50 nM of the gga-19b-3p mimic when compared with those not treated with the gga-19b-3p mimic.