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. 2015 Nov 4;6:1230. doi: 10.3389/fmicb.2015.01230

FIGURE 2.

FIGURE 2

Naltrexone blocks the heroin-mediated enhancement of HIV. Seven-day cultured macrophages were incubated with or without heroin at the indicated concentrations for 6 h prior to HIV Bal, JRFL, or JAGO infection. Naltrexone (10-6 M) was added to the macrophages cultures for 1 h prior to heroin (10-7 M) treatment. HIV RT activity was determined at day 12 postinfection. Cell culture supernatant was collected at the indicated time points and subjected to RT assay to detect the HIV RT activity. The data shown is the mean ± SD of triplicate cultures representative of three experiments using cells from three different donors (∗∗p < 0.01, heroin vs. heroin + naltrexone).