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. 2015 Nov 4;6:1230. doi: 10.3389/fmicb.2015.01230

FIGURE 4.

FIGURE 4

Naltrexone blocks the heroin action on HIV restriction miRNAs. Naltrexone (10-6 M) was added to macrophage cultures for 1 h prior to heroin treatment. Total cellular RNA was then extracted from the cell cultures 6 h post-treatment and subjected to realtime RT-PCR for miRNA expression: miRNA-28 (A), miRNA-125b (B), miRNA-150 (C), and miRNA-382 (D). The data shown is the mean ± SD of triplicate cultures representative of three experiments using cells from three different donors (∗p < 0.05, ∗∗p < 0.01, heroin vs. control, or heroin + naltrexone).