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. 2015 Nov 4;6:1230. doi: 10.3389/fmicb.2015.01230

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Copyright © 2015 Wang, Ma, Li, Zhou, Geller, Adler, Peng, Zhou, Zhou and Ho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heroin users have decreased levels of HIV restriction miRNAs. Total RNA was exacted from macrophages derived from monocytes of heroin addicts and normal control subjects. miRNA-28 (A), miRNA-125b (B), miRNA-150 (C), miRNA-382 (D), and miRNA-223 (E) was amplified using specific primers and quantified by real time RT-PCR. The results are expressed as relative transcript abundance of miRNA/GAPDH.