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. 2015 Nov 4;5:16074. doi: 10.1038/srep16074

Figure 4. Changes in TtcA before and after rac excision.

Figure 4

(A) Excision of rac was induced by overproduction of excisionase and integrase proteins in E. coli K-12 BW25113. (B) Rac excision caused a change of four amino acids of TtcA at the N-terminus. Numbers on the top indicate the amino acid position in TtcA. (C) The nucleotide sequence of the left and the right attachments of rac before excision (attL and attR) as well as the attachment site after rac excision (attB) in E. coli K-12 BW25113. Letters labeled under the attB sequence indicate the partial TtcA’ amino acid sequence. Numbers on the bottom indicate the amino acid position in TtcA’. (D) The nucleotide sequence of the two attachments sites (attL and attR) of rac-like prophage, CP-933R in E. coli O157:H7 Str. EDL933 strain and Sp10 in E. coli O157:H7 Str. Sakai strain. Arrows indicate the translational start of TtcA.