Table 1. Bacterial strains and plasmids used in this study.
| Bacterial strains/Plasmids | Description | Source |
|---|---|---|
| BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 54 |
| BL21(DE3) | F-ompT hsdSB(rB-mB-) gal dcm λ(DE3) Ω PtacUV5::T7 polymerase | Novagen |
| ΔxisR | xisR (ydaQ) deletion mutant derived from BW25113, KmR | 54 |
| ΔttcA | ttcA (ydaO) deletion mutant derived from BW25113, KmR | 54 |
| Δhns | hns deletion mutant derived from BW25113, KmR | 54 |
| ΔrpoS | rpoS deletion mutant derived from BW25113, KmR | 54 |
| Δ9 | Δrac ΔCP4-57 ΔCPS-53 ΔDLP12 ΔQin Δe14 ΔCP4-6 ΔCPZ-55 ΔCP4-44 ΔKmR derived from BW25113 | 7 |
| ΔDLP12 | Prophage DLP12 deletion mutant derived from BW25113 | 7 |
| Δrac | Prophage rac deletion mutant derived from BW25113 | 7 |
| ΔCP4-44 | Prophage CP4-44 deletion mutant derived from BW25113 | 7 |
| ΔCPZ-55 | Prophage CPZ-55 deletion mutant derived from BW25113 | 7 |
| ΔCP4-57 | Prophage CP4-57 deletion mutant derived from BW25113 | 7 |
| ΔQin | Prophage Qin deletion mutant derived from BW25113 | 7 |
| ΔCP4-6 | Prophage CP4-6 deletion mutant derived from BW25113 | 7 |
| Δe14 | Prophage e14 deletion mutant derived from BW25113 | 7 |
| ΔCPS-53 | Prophage CPS-53 deletion mutant derived from BW25113 | 7 |
| Plasmids | ||
| pCA24N | CmR; lacIq, IPTG inducible expression vector in E. coli | 55 |
| pLP170 | CarR, vector used for cell lysis assay in BW25113 | 59 |
| pCA24N-xisR | CmR; lacIq, vector for expressing xisR in E. coli host | This study |
| pCA24N-ttcA | CmR; lacIq, vector for expressing ttcA in E. coli host | This study |
| pCA24N-ttcA’ | CmR; lacIq, vector for expressing the ttcA variant in E. coli host | This study |
| pET28b | KmR, expression vector | Novagen |
| pET28b-xisR | KmR, lacIq, pET28b PT7-lac:: xisR with six histidines tagged at the N-terminus | This study |
Note: KmR, CmR and CarR indicate kanamycin, chloramphenicol and carbenicillin resistance, respectively.