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. 2015 Nov 4;5:16083. doi: 10.1038/srep16083

Figure 1. Increased CUG-BP1 protein level and decreased ASI(+) isoform in denervation-induced skeletal muscle atrophy of mice.

Figure 1

(a) Western blotting results show CUG-BP1 protein level is increased in the process of denervation-induced atrophy in TA muscle. (b) mRNA expression level of CUG-BP1 and RyR1 ASI alternative splicing determined by RT-PCR in the process of denervation-induced atrophy in TA muscle. (c) Real time PCR analysis of RyR1 ASI(+) proportion in 3, 7, 10, 14 days after denervation. (d) The correlation between the increased RyR1 ASI(+) isoform and the developmental loss of CUB-BP1 protein during three weeks after postnatal. To keeping the clarity and conciseness, the cropped gels and blots were presented in the current and following figures. The full-length gels/blots are included in the supplementary information. Three independent experiments were performed and one representative result was presented. Data are presented as the means ± s.e.m. **P < 0.01 and ***P < 0.001 by two-tailed Student’s test.