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. 2015 Nov 4;5:16083. doi: 10.1038/srep16083

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Copyright © 2015, Macmillan Publishers Limited

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(a) Protein level of CUG-BP1 in control and neurogenic atrophic human skeletal muscle was detected by western blot assay (upper panel), the statistic results of densitometry analysis showed that the expression of CUG-BP1 was enriched in atrophic human skeletal muscle (lower panel). (b) Analysis of RyR ASI splicing pattern was performed by RT-PCR and then separated on an 8% polyacrylamide gel, the statistic results showed the proportion of RyR1 ASI(+) isoform was decreased accordingly (lower panel). Data are presented as the means ± s.e.m from three control and three atrophic human gastrocnemius muscle samples; *P < 0.05 and **P < 0.01 by two tailed Student’s test.