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. 2015 Nov 4;5:16083. doi: 10.1038/srep16083

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Copyright © 2015, Macmillan Publishers Limited

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(a) Dsred tagged mouse CUG-BP1 cDNA was over-expressed in FDB muscle. Ten days after electroporation, over-expression of CUG-BP1 was verified by western blot. The proportion of RyR1 ASI(+) isform was decreased from 49 ± 2% to 30 ± 3%. (b) CUG-BP1 knock-down assay in denervated FDB. The protein level of CUG-BP1 was inhibited in both shRNA assays; RyR1 ASI(+) isoform was increased from 27 ± 3% to 39 ± 4% in shRNA 1 and 41 ± 3% in shRNA 2 samples. (c) RIP analysis in undifferentiated C2C12 cells. IP was performed using antibodies against CUG-BP1, and RT-PCR assay using primers targeting adjacent intron of RyR1 exon 70 as indicated. Beta-actin was used as input control. (d) Real time PCR assay using the same primers targeting adjacent intron of RyR1 exon 70. Data are presented as the means ± s.e.m.; *P < 0.05.