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. 2015 Nov 4;5:16083. doi: 10.1038/srep16083

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Copyright © 2015, Macmillan Publishers Limited

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(a) Representative images of FDB muscle fibers transfected with control and CUG-BP1 shRNA. Images were taken at day 10 after denervation with a total 400 × magnitude. (b) Statistic result of FDB myofiber diameters transfected with CUG-BP1 shRNA. n = 120 from 4 mice. (c) Relative mRNA levels of muscle atrophy-related genes in shRNA-CUG-BP1 denervated muscles. The mRNA levels of LC3, Bnip3, and Atrogin-1 in denervated shRNA-CUG-BP1 muscles were decreased to 38.7 ± 4.6%, 31.0 ± 5.4% and 57.5 ± 8.7% of those in the control shRNA muscles. Data are presented as the means ± s.e.m. *P < 0.05, **P < 0.01 and ***P < 0.001 by two-tailed Student’s test.