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. 2015 Nov 4;5:16094. doi: 10.1038/srep16094

Figure 5. Pro-inflammatory cytokine levels were reduced in S. typhimurium-infected mice pretreated with MET-1.

Figure 5

(a) Serum cytokine levels were measured 48 hours post-infection using a Bio-Plex Pro mouse cytokine magnetic bead kit. Of the cytokines measured, TNF-α, IL-1β, IL-12p40, IL-12p70, GM-CSF, eotaxin, MIP-1β, and MCP-1 were all significantly reduced in infected mice pretreated with MET-1 (MET-1 + S. Tm.) compared to infected mice pretreated with vehicle control (VC + S. Tm.). There were no significant differences in cytokine concentrations between uninfected mice pretreated with MET-1 or vehicle control (data not shown). Data were analyzed using a 1-way ANOVA with Bonferroni correction, n = 7 for each group (*p < 0.05, **p < 0.01, ***p < 0.001). (b) Cecal TNF-α expression was attenuated in S. typhimurium-infected mice pretreated with MET-1, as determined by quantitative RT-PCR (qRT-PCR) was performed in triplicate. The relative TNF-α expression was normalized to an endogenous control (GAPDH expression). The relative expression of TNF-α was attenuated in infected mice pretreated with MET-1 (MET-1 + S. Tm.) compared to infected mice pretreated with vehicle control (VC + S. Tm.). The level of expression in MET-1 + S. Tm. mice was not significantly different from uninfected mice pretreated with vehicle control (VC) or MET-1. Data were analyzed using a 1-way ANOVA with Newman-Keuls multiple comparison test, n = 4 for each group (*p < 0.05).