Figure 3.

Conservation of amino acids for identified causal mutations. (A–D, F–I) For each conserved gene, both the wild-type and mutant C. elegans protein sequences were aligned against homologs from other species (Cel = C. elegans; Sce = S. cerevisiae; Dme = D. melanogaster; Dre = D. rerio; Mmu = M. musculus; Hsa = H. sapiens). The level of sequence conservation has been highlighted using Jalview’s Color by Percentage Identity function (Waterhouse et al. 2009). (E) Schematic of the ndg-4 transcript, with the mutated splice donor site highlighted with an asterisk (*).