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. 2015 Aug 26;5(11):2241–2255. doi: 10.1534/g3.115.021451

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Copyright © 2015 Lowry et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rachis extension in drp-1(or1393ts) mutants and GFP::DRP-1 localization. (A) The rachis appears to extend to the most mature oocyte (arrowheads) in an adult drp-1(or1393ts) mutant gonad, as detected using mCherry::PH to mark the membranes. (B) ANI-2::GFP localizes to the surface of the rachis (Maddox et al. 2005), which terminates just after the turn in wild-type hermaphrodites. The rachis again extends proximally in an adult drp-1(or1393ts) mutant gonad (arrowheads). (C and D) GFP::DRP-1 (see Materials and Methods) does not localize to openings to the rachis in the distal gonad or to the rachis more proximally, arguing against a direct role in oocyte cellularization. (C) An orthogonal view of the meiotic germ cell openings to the rachis of wild-type strains carrying either ANI-2::GFP or GFP::DRP-1. GFP::DRP-1 does not localize to the germ cell opening (arrows). (D) A view in the plane of the meiotic germ cell openings from the rachis surface. Solid white lines in (C) and (D) mark insets, and dashed white lines indicate individual germ cells with openings to rachis.