Table 2. Causal mutations for 10 Osm/Ste mutants identified by whole genome sequencing and complementation tests.
| Allele | Transcript Name | Codon Mutated | Amino Acid Change | Nucleotide Change | Transcript Nucleotide (Spliced) | Average Depth of Coverage |
|---|---|---|---|---|---|---|
| atx-2 (or821ts) | D2045.1a | 175 | S > P | T > C | 523 | 75.3X |
| sqv-8 (or888ts) | ZK1307.5 | 187 | L > F | C > T | 559 | 24.2X |
| crn-3 (or959ts) | C14A4.4 | 318 | L > F | C > T | 954 | 25.6X |
| rpl-7 (or990ts) | F53G12.10 | 165 | I > S | T > G | 494 | 30.4X |
| ndg-4 (or1088ts) | F56F3.2 | —a | — | C > T | — | 25.6X |
| vps-15 (or1235ts) | ZK930.1 | 284 | L > P | T > C | 851 | 17.1X |
| rpl-7 (or1247ts) | F53G12.10 | 165 | I > N | T > A | 494 | 32.7X |
| drp-1 (or1393ts) | T12E12.4 | 39 | G > E | G > A | 116 | 28.3X |
| abtm-1 (or1400ts) | Y74C10AR.3 | 621 | R > P | C > G | 1862 | 35.0X |
| ippk-1 (or1572ts) | Y17G7B.13 | 137 | V > F | G > T | 409 | 32.3X |
Nature and location of each mutation and the average depth of sequencing coverage are shown for each mutant.
a
The mutation in ndg-4(or1088) affects a splice site donor at the end of exon IV.