Figure 1.

AMP expression in adult heads and bodies is increased following irradiation of larvae. For each dose of radiation during the late third instar, expression of mRNAs encoding six different AMPs in heads (A, B) and bodies (C, D) of 5-d-old and 15-d-old adults was measured by qRT-PCR analysis. Wild-type male Canton-S flies were used for these experiments. Bars represent mean expression of each AMP mRNA (3–5 trials each) normalized to mean expression of the corresponding RNA in nonirradiated, age-matched controls (fold-induction). Error bars are SEM. Rp49 was used as the reference gene. See Table S1 for primers used. *P < 0.05, **P < 0.05, and ***P < 0.01 based on Student’s t-test comparing relative expression in 40 Gy or 30 Gy samples to 10 Gy samples.