Figure 2.

AMP-GFP reporter expression is increased in adult brains following irradiation of larvae. Following exposure of late third instar larvae to a 40-Gy dose of radiation, surviving adults were aged for 5 d after eclosion and brains were dissected for confocal microscopy. (A–Q) Confocal stacks are shown of Metchnikowin-GFP and Attacin-GFP brains immunostained for GFP (green, A–D), the neuronal (nuclear) marker Elav (red, E–H), and the glial (nuclear) marker Repo (blue, I–L). All markers are shown in the overlay (M–P). Images are of the optic lobe, as shown by the dashed box in (R). The indicated region of (P) (Attacin-GFP) is shown at higher magnification in the inset (Q). Brains from adults that were irradiated during larval development show increased GFP expression (C, D) compared to brains from adults that were not irradiated as larvae (A, B). Some of the GFP+ puncta are in close proximity to nuclei that are Repo+ (arrowheads in Q) or Elav+ (* in Q). Scale bars are 20 μm; 7–10 brains of each genotype for each condition were analyzed.