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. 2015 Sep 10;5(11):2429–2439. doi: 10.1534/g3.115.022327

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Copyright © 2015 Brown et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Robo1^ΔIg1 is sensitive to downregulation by Comm. (A–D) Stage 16 embryos stained with anti-HRP (magenta) and anti-HA (green) antibodies. Bottom images show HA channel alone from the same embryos. Embryos carrying one copy of the robo1::robo1 transgene (A) display normal expression of the HA-tagged Robo1 receptor (arrow), whereas sibling embryos carrying one copy of robo1::robo1 along with UAS-Comm (B) display a strong reduction in HA staining along with a corresponding increase in midline crossing, leading to a slit-like midline collapse phenotype (arrow with asterisk). Levels of Robo1^ΔIg1 expressed from the robo1::robo1^ΔIg1 transgene are similarly reduced in the presence of ectopic Comm expression (D) compared to sibling embryos without UAS-Comm (C). Pairs of sibling embryos shown here (A–B and C–D) were stained in the same tube and imaged under identical conditions to allow an accurate comparison of HA levels between embryos. All embryos (A–D) carry two copies of elav-GAL4.