Table 2. Ectopic midline crossing defects in robo1 mutant and rescue backgrounds.
| Genotype | % Segments with Ectopic FasII Crossing | N (Segments/Embryos) |
|---|---|---|
| robo11/+ | 5.2 | 96/12 |
| robo11/robo11 | 100 | 80/10 |
| GAL4/UAS rescue | ||
| robo11/robo11; elav-GAL4/UAS-Robo1 | 3.8 | 104/13 |
| robo11/robo11; elav-GAL4/UAS-Robo1ΔIg1 | 100 | 104/13 |
| robo1 genomic rescue | ||
| robo11, robo1::robo1/robo11, robo1::robo1 | 2.9 | 104/13 |
| robo11, robo1::robo1ΔIg1/ | 100 | 104/13 |
| robo11, robo1::robo1 ΔIg1 |
Stage 16 and 17 embryos were stained with anti-FasII and abdominal segments from dissected ventral nerve cords were scored for ectopic midline crossing of FasII-positive axons.