Table 1. Genes associated with carbohydrate-active enzyme (CAZy) activity in the set of overexpressed genes in yeast or mycelial phase in Ophiostoma novo-ulmi.
| Growth Phase | O. novo-ulmi Genes | Overexpression (logFCa Y vs. LM) | Overexpression (logFC Y vs. SM) | Description | CAZyb |
|---|---|---|---|---|---|
| Yeast | OphioH327g3368 | 3.15 | 2.65 | Mannan endo-1,6-alpha-mannosidase DCW1 | GH76 |
| OphioH327g0928 | 2.65 | 2.94 | Alpha-L-fucosidase | GH29 | |
| OphioH327g0277 | 1.74 | 1.16 | Glycoside hydrolase 15-related | GH15 | |
| OphioH327g0547 | 2.41 | 4.42 | 1.4-beta-D-glucan cellobiohydrolase C | GH6 | |
| OphioH327g1070 | 1.38 | 0.92 | Protein phosphatase regulatory subunit | CBM21 | |
| OphioH327g5673 | 1.28 | 0.98 | Mannan endo-1.6-alpha-mannosidase DCW1 (Defective cell wall 1) | GH76 | |
| OphioH327g4175 | 1.55 | 1.95 | Endochitinase 2 | GH18 | |
| OphioH327g8225 | 1.55 | 1.59 | Chitin synthase D (GT2 family) | GT2 | |
| OphioH327g2068 | 1.78 | 3.19 | Xylosidase/arabinosidase (Beta-xylosidase) | GH43 | |
| OphioH327g0647 | 3.65 | 3.6 | Exoglucanase 1 | GH7 | |
| OphioH327g6063 | 0.94 | 0.91 | UDP-N-acetylglucosaminyltransferase | GT41 | |
| Mycelium | OphioH327g0635 | 2.89 | 3.18 | GDSL-like Lipase/Acylhydrolase | CE12 |
| OphioH327g3383 | 1.88 | 1.20 | Carbohydrate-binding module family 52 protein | CBM52 | |
| OphioH327g7070 | 1.81 | 1.60 | Endochitinase 1 (Complement-fixation antigen) | GH18 | |
| OphioH327g6213 | 1.42 | 1.48 | Glycosyltransferase family 22 protein | GT22 | |
| OphioH327g7370 | 4.60 | 3.68 | Endo-N-acetyl-beta-D-glucosaminidase | GH18 | |
| OphioH327g4365.1 | 1.38 | 1.30 | Chitin synthase 3 | GT2 | |
| OphioH327g3285 | 1.30 | 1.64 | Protein mannosyltransferase 1 | GT39 | |
| OphioH327g6864 | 1.41 | 1.66 | Beta-glucanosyltransferase gel2 | GH72 | |
| OphioH327g7251 | 3.66 | 3.85 | Putative cellulose synthase 3 (Cyclic di-GMP-binding domain) | GT2 | |
| OphioH327g5590 | 4.86 | 5.79 | Rhamnogalacturonase | GH28 | |
| OphioH327g8280 | 1.24 | 1.42 | UDP-glucose:glycoprotein glucosyltransferase | GT24 | |
| OphioH327g5097 | 1.10 | 1.20 | Dolichol-phosphate mannosyltransferase | GT2 | |
| OphioH327g2358 | 0.90 | 1.39 | Oligosaccharyl transferase subunit | GT66 | |
| OphioH327g3447 | 1.73 | 1.99 | EGF domain-specific O-linked N-acetylglucosamine transferase | GT61 | |
| OphioH327g4450 | 0.91 | 1.03 | Mannosyltransferase pmti | GT39 | |
| OphioH327g0970 | 0.93 | 1.24 | Chitin synthase 1 (Class-III chitin synthase 3) | GT2 |
a
LogFC, log2 of fold change between yeast and mycelium phases; Y, yeast phase; LM, mycelium grown in liquid medium; SM, mycelium grown in Petri dishes. FDR ≤1%.
b
GH, glycoside hydrolases; GT, glycosyltransferases; CBM, carbohydrate-binding module. Numbers associated with CAZy types refer to the family number.