Figure 2. Single-conidium trapping and compartmentalized hyphal growth.
(a) A single conidium of N. Crassa hydrodynamically trapped at the conidial trapping site. The conidia are loaded at a concentration of 2 × 105 cells/mL in 1× Vogels’ salts and a flow rate of 0.4 μL/min, with the medium outlet valve open while the waste valve closed. Scale bar, 10 μm. (b) The compartmentalized hyphal extension of N. Crassa along the hyphal growth channels (2.5 mm × 10 μm × 10 μm) due to the closure of isolation valve. The hyphae are cultivated under constant flow of 10% glucose in 1× Vogels’ salts solution at 0.3 μL/min. Scale bar, 100 μm.