Figure 5. Three major forms of nuclear movement in subapical compartments.

(a) Oscillation (back-and-forth motion). The circle indicates the oscillated nucleus. (b) Bypassing of a fluorescently strong nucleus by a fluorescently weak nucleus. The circle indicates the two nuclei. (c) Passage through septa of a nucleus. The arrow indicates the passing nucleus. All images are from Supplementary Video 3. Scale bar, 10 μm. In the experiment, N. Crassa strain NMF617 expressing histone H1-RFP is grown in 2.5 mm × 10 μm × 10 μm channels under the constant flow of 10% glucose in 1× Vogels’ salts solution at 0.3 μL/min.