Skip to main content
. 2015 Nov 4;5:16111. doi: 10.1038/srep16111

Figure 6. Long-term monitoring of gene expression dynamics on the microfluidic chip.

Figure 6

(a,b) The expression level of xylose transporter-GFP of each hyphal compartment (from conidial end to tip; left panels) and entire hypha (right panels) in two representative hyphae at 30, 48, 60 and 72 h. The hyphae are constantly grown in 10% glucose. (c) Two representative hyphae with fluorescent gradient. Scale bar, 10 μm. (d,e) The expression level of xylose transporter-GFP of each hyphal compartment (from conidial end to tip; left panels) and entire hypha (middle panels) in typical hyphae, and representative images of the same compartments over time (right panels) at 30, 45, 52, 60 and 72 h, in response to (d) glucose starvation at 48 h and replenishment at 62 h and (e) carbon source switching from 10% glucose (Glu) to 10% xylose (Xyl) at 48 h. Scale bar, 10 μm. In the experiments, N. Crassa strain XEB 12 expressing xylose transporter-GFP is grown in 2.5 mm × 10 μm × 10 μm channels under the constant flow of different media at 0.3 μL/min.