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. 2015 Aug 6;4(11):834–845. doi: 10.1016/j.molmet.2015.07.009

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Basal myokine secretion by human skeletal myotubes is affected by circadian clock disruption. Myoblasts were transduced with the Bmal1-luc lentivector, differentiated into myotubes, transfected with either siControl or siClock siRNA, and subjected to continuous perifusion with parallel bioluminescence recording. Concentrated perifusion samples were assessed by multiplex analysis. 2 technical duplicates from 3 biological samples were analyzed for each time point, and normalized to the total DNA content. Basal secretion profiles (mean + SEM) in the presence or absence of a functional clock are shown for (A) MCP-1, (B) M-CSF, and (C) VEGF.