Figure 3.

1 week of HFD promotes proliferation of AdPCs. (A, B, E) SVF was isolated from epididymal VAT of 8 to 10 week old C57BL/6J mice fed 1 week of either chow or HFD. n = 10–11 per group. (A) Flow quantitation of MCP-1^hi and MCP-1^mid cells per mouse (paired eVAT depots). (B) Flow quantitation of total AdPCs per mouse (paired eVAT depots). (C–D) 7 to 8 week old male C57BL/6J mice were fed standard chow or HFD for 1 week, and were injected with BrdU (bromodeoxyuridine) 5 times over the course of the diet. n = 11. (C) Time course of BrdU injections during 1 week of diet. (D) Percentage of BrdU uptake in AdPCs. (E) Flow quantitation of CD24⁺ and CD24⁻ AdPCs per mouse (paired eVAT depots). Shown are mean values ± SEM, **p < 0.01, ns = p > 0.05.