Figure 8.

Human omental adipocyte progenitor cells express abundant MCP-1: an effect marked by high levels of CD44. (A–E) Subcutaneous and omental adipose tissue were collected during bariatric surgery from consenting human subjects (n = 14), and were processed to SVF cells for flow cytometry. (A) Percentage of SVF cells that were CD45⁻CD31⁻CD34⁺CD90⁺CD44⁺ adipocyte progenitor cells in human omental and subcutaneous adipose tissue. (B) Percentage of AdPCs that were MCP-1⁺ in omental and subcutaneous adipose tissue. (C) Representative plot depicting the heterogeneity of CD44 staining in CD45⁻CD31⁻CD34⁺CD90⁺ cells from omental VAT, and MCP-1 intracellular staining in each CD44 subset. 1 = CD44⁻, 2 = CD44^lo, 3 = CD44^hi (D) Percentage of AdPCs that are MCP-1⁺ as a function of CD44^hi and CD44^lo status in omental and subcutaneous adipose tissue. (E) Correlation of gMFI of MCP-1 with gMFI of CD44 in CD45⁻CD31⁻CD34⁺ CD90⁺ AdPCs in both omental and subcutaneous adipose tissue. Shown are mean values ± SD. (F, G) SVF was isolated from epididymal VAT of 8 to 10 week old C57BL/6J mice fed 1 week of either chow or HFD. n = 4. (F) Percentages of CD45⁻CD31⁻CD34⁺CD29⁺Sca-1⁺CD24⁻ AdPCs that are MCP-1^hi as a function of CD44^hi and CD44^lo status, in chow-fed and HFD-fed mice. (G) Correlation of gMFI of MCP-1 with gMFI of CD44 in CD45⁻CD31⁻CD34⁺CD29⁺Sca-1⁺CD24⁻ AdPCs, fed chow or HFD. Shown are mean values ± SEM, *p < 0.05, **p < 0.01, ****p < 0.0001, ns = p > 0.05. gMFI = Mean Fluorescence Intensity, using geometric mean.