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. 2015 Aug 14;4(11):811–822. doi: 10.1016/j.molmet.2015.08.001

Figure 7.

Figure 7

PGC-1s regulate expression of lipolytic enzymes important for acyl-glycerol metabolism in response in β-cells. (A) Western blot of lipolytic enzymes in primary islets (representative blot of n = 4). (B) qPCR of primary islets from Pgc1 KO mice and WT fl/fl littermate controls (n = 10). (C) Acyl-glycerol content of primary islets (n = 4). (D) Radio-labeled acyl-glycerol species incorporated by primary islets, separated by thin layer chromatography (MAG-Monoacylglycerol, DAG-Diacylglycerol, TG-Triglycerides, CE-Cholesterol ester) (n = 5–8). Data are means ± SEM, *p < 0.05, **p < 0.01 compared to WT fl/fl controls (E) qPCR analysis of genes following overexpression of Pgc-1α1, Pgc-1α4, or Pgc-1β in INS1 cells (n = 3). Data are means + SD, *p < 0.05 compared to cells infected with viral vector (Ad-control).