Table 1. Monocyte/Macrophage Staining Panel Designed for Flow Cytometry.
| Antibody | Clone | Fluorophore | Manufacturer | Location |
|---|---|---|---|---|
| MAC387 | MAC387 | FITC | Abcam (Cambridge, UK) | Intracellular |
| CD163 | GHI/61 | PE | BioLegend (San Diego, CA, USA) | Surface |
| CD68 | KP1 | PerCP-Cy5.5 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | Intracellular |
| CD45 | D058-1283 | APC | BD Biosciences | Surface |
| CD3 | SP34-2 | Pacific Blue | BD Biosciences | Surface |
| HLA-DR | G46-6 | Alexa Fluor 700 | BD Biosciences | Surface |
| CD14 | RMO52 | ECD | Beckman Coulter (Brea, CA, USA) | Surface |
| CD8 | B9.11 | PE-Cy5 | Beckman Coulter | Surface |
| CD16 | 3G8 | PE-Cy7 | BD Biosciences | Surface |
| CD20 | 2H7 | APC-H7 | BD Biosciences | Surface |
| ARD | – | LIVE/DEAD Yellow | Invitrogen (Carlsbad, CA, USA) | – |
APC, Allophycocyanin; ECD, energy-coupled dye.