Figure 6.

Sirt6BAC mice exhibit enhanced insulin-induced pAKT in gastrocnemius. Immunoblot and quantification of Akt (Thr³⁰⁸) phosphorylation status relative to total AKT (AKT) in gastrocnemius muscle 10 min after an intraperitoneal bolus of insulin (5 U/kg) or saline in (A) wild-type vs. Sirt6BAC mice and (B) wild-type vs. Sirt6BAC; Sirt6^−/− mice. All mice were fasted 5 h before insulin or saline injection. All mice were age-matched and fed on a standard diet. Error bars represent s.e.m. Statistical analyses were done using two-tailed unpaired Student's t test. *P < 0.05; ***P < 0.001.