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. 2015 Nov 2;6:8728. doi: 10.1038/ncomms9728

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(a) Structured illumination microscopy (3D SIM) images of B. subtilis cells stained with Nile red (upper panel) and expressing TlpA-GFP (middle panel). Merged image shown in lower panel. (b) Phase-contrast image (upper left panel), confocal fluorescence image (upper right panel) and 3D reconstruction of optical sectioning of cells expressing TlpA-GFP (lower panel). (c) Localization of TlpA-GFP in the absence of DivIVA. 3D SIM images of Nile red membrane stained divIVA deletion mutant (upper panel) expressing TlpA-GFP (middle panel), and merged image (lower panel) are depicted. In the absence of DivIVA, B. subtilis cells frequently undergo multiple adjacent cell division events60,61. Strains used: (a,b) B. subtilis HS49 (Δmcp Pxyl-tlpA-gfp) and (c) B. subtilis HS52 (Δmcp Pxyl-tlpA-gfp ΔdivIVA). Scale bar, 3 μm.