Figure 4.

Knockdown of NRAS or E2F2 enhances the sensitivity of SGC-7901/DDP cells to cisplatin. (a) The mRNA and protein levels of NRAS and E2F2 were all reduced after transfection with siRNAs in SGC-7901/DDP cells. (b) The cell survival was examined by MTS assay after siRNA transfection. (c) Knockdown of NRAS or E2F2 enhances cisplatin-induced cell apoptosis. The transfected cells incubated with 5 mg/L DDP for 48 h and the apoptotic rates were evaluated by Annexin V-FITC and PI staining and analyzed with FACS. *P < 0.05; **P < 0.01