Figure 2.

Combined AZD5582 and TRAIL synergize and induce caspase-dependent cell death in some breast cancer cell lines. (a) Four AZD5582/TRAIL-resistant and four sensitive cell lines, as indicated, were treated with 10 nM AZD5582, 10 ng/ml TRAIL or a combination for the indicated period of time and 20 μg of protein lysate was used for each lane and PARP was detected by western blotting. (b) Caspase-activity was quantitated using Nuc-view visualised using Incucyte in four sensitive cell lines treated with 0.1 nM AZD5582 and 10 ng/ml TRAIL over the period of 45 h. Error bars represent S.E.M. of triplicates. (c) An example of cell morphology changes occurring to MDA-MB-231 cells in response to 0.1 nM AZD5582, 10 ng/ml TRAIL and a combination over the 24-h time course. (d) Cell viability was evaluated using proliferation-cell death assay following treatment with 0.1 nM AZD5582 and 10 ng/ml TRAIL in the presence and absence of a pan-caspase inhibitor OPh-QVD, over 24 h