Figure 5.

Overexpression of RCAN1 suppressed the growth of lymphoma xenograft tumor in SCID mice. (a) RCAN1 decreased incidence of lymphoma xenograft in SCID mice. The x-axis indicated the days after transplantation. (b) The tumor volume was calculated from the tumor diagram measured every day. The x-axis indicated the days after transplantation. P=0.0008 by two-way ANOVA. (c) The mice were killed and tumors were dissected. The tumor tissues were homogenated and separated with SDS-PAGE. Anti-IκBα antibody was used to detect endogenous IκBα and β-actin was used as loading controls. (d) Quantification of c. Values represent mean±S.E.M.; n=8; *P=0.0013 by Student's t-test. (e) The tumor volume of 1000 mm³ was defined as the censored event in the log rank Mantel–Cox test (P=0.015), which was analyzed with SPSS17.0 statistics software (Armonk, NY, USA)