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. 2015 Oct 22;6(10):e1930. doi: 10.1038/cddis.2015.263

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of salinomycin, nigericin and valinomycin on lymphocytes. Jurkat leukemic T cells (a and b) and human primary B-CLL cells (c) were incubated for 24 h with different concentrations of the compounds, as indicated. Cell survival (a) was measured by MTT assay; values are expressed as the average percentage of cell survival compared with untreated cells ± S.E.M. (n=5). Cell death (b and c) was tested using FACS by the staining with FITC-Annexin V and propidium iodide. Staurosporine (Stauro or ST) was used as positive control. Indicated values refer to the percentage of dead cells ± S.E.M. (n=14). Statistically significant differences (P<0.05) are indicated by asterisks