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. 2015 Oct 22;6(10):e1943. doi: 10.1038/cddis.2015.295

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Co-localization of endogenous Pur-alpha with FUS proteins carrying single ALS-associated mutations. (a) HeLa cells transfected with plasmids encoding Flag-FUSWT or FUS carrying the indicated single C-terminal mutations were stained by immunofluorescence with anti-Pur-alpha, anti-Flag, and anti-TIAR antibodies (bars=10 μm). (b) Localization of endogenous FUS and Pur-alpha in mouse spinal cord as demonstrated by the counterstain with antibodies directed against the neuronal marker NeuN (bars=20 μm)