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. 2015 Oct 22;6(10):e1943. doi: 10.1038/cddis.2015.295

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of Pur-alpha and FUSMM expression on eIF2-alpha phosphorylation of HEK293-starved cells were transfected with HA-Pur-alpha, Flag-FUSWT, or Flag-FUSMM. eIF2-alpha phosphorylation was evaluated by western blotting with anti-p-eIF2-alpha antibody. In starved HEK293 cells the expression of HA-Pur-alpha determines a strong induction of eIF2-alpha phosphorylation as well as Flag-FUSMM, if compared with cells expressing Flag-FUSWT or untransfected cells. The lower panel shows band quantification generated with ImageJ analysis package. Data are presented as the ratio of phospho-eIF2-alpha to total eIF2-alpha signal and are expressed in arbitrary units (AU). Values are reported as mean±S.D.; n=3. Statistical significance was evaluated with Student's t-test (** indicate values significantly different from controls with P-value <0.001)