Table 1.
Method validation for quantification of ART in rat plasma
| QC (ng/mL) | Precision and accuracy (%) | Stability (RE%) | Recovery (%) | Matrix effect (n = 5) Mean (%)/RSD% |
||||
|---|---|---|---|---|---|---|---|---|
| Inter-day RSD/RE |
Intra-day RSD/RE |
Pre-T | Post-T | Freeze–thaw for three times | ||||
| RT 6 h |
RT 24 h |
RT 24 h |
||||||
| 0.5 | 8.51/1.24 | 6.57/1.24 | −4.00 | −2.00 | 2.00 | 2.00 | 57.30 | 98.0/1.70 |
| 10 | 1.41/6.75 | 6.67/−1.90 | 3.20 | −0.80 | −8.20 | 6.80 | 73.30 | 88.0/2.70 |
| 160 | 2.97/7.81 | 6.99/0.67 | −6.25 | −3.00 | −4.38 | 4.38 | 68.90 | 66.0/3.10 |
RT room temperature, Pre-T Pre-treatment, Post-T post-treatment